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101.
Rapid salinity changes affect the survival and physiology of a penaeid prawn: Implications of flood events on recruitment to the fishery 下载免费PDF全文
K. J. Tyler A. Becker N. A. Moltschaniwskyj M. D. Taylor 《Fisheries Management and Ecology》2017,24(6):478-487
Estuaries act as nurseries for many penaeid prawns, but these habitats are highly susceptible to salinity decline through flooding. The rate of salinity decline and duration of exposure to non‐optimal salinity may affect survival and subsequent recruitment of prawns to the fishery. This study aimed to determine the effect of salinity fluctuations observed in local estuaries during flood events using a novel dilution approach. Mortality of juvenile Melicertus plebejus (Hess) was assessed after 24 hr exposure to 24 rates of salinity decline ranging from 0.01% to 20% per hr. After the salinity decline, prawns were held at the final salinities for five days before again assessing mortality as well as aerobic metabolic rate and prawn water content. Salinity decline from 36 to ~0.8 led to 50% mortality, but continued exposure to low salinity for five days increased mortality at this salinity to 99% and shifted the 50% mortality point to salinity ~5. Aerobic metabolic rate and water content data suggested the cause of mortality due to exposure to salinities < 5 was osmoregulatory failure. Rapid salinity declines over 24 hr and sustained low salinity due to flooding could compromise the survival of juvenile prawns, potentially reducing recruitment to the fishery. 相似文献
102.
Investigations into the temporal development of epitheliocystis infections in brown trout: a histological study 下载免费PDF全文
B Vidondo L Vaughan J‐F Rubin H Segner S Samartin H Schmidt‐Posthaus 《Journal of fish diseases》2017,40(6):811-819
Epitheliocystis in Swiss brown trout (Salmo trutta) is a chlamydial infection, mainly caused by Candidatus Piscichlamydia salmonis and Candidatus Clavichlamydia salmonicola. To gain a better understanding of the temporal development of infections in wild brown trout, we investigated epitheliocystis infections during the course of the summer and autumn months of a single year (2015), and compared this to sampling points over the span of the years 2012–2014. The survey focused on tributaries (Venoge and Boiron) of the Rhone flowing in to Lake Geneva. When evaluated histologically, epitheliocystis infections were found throughout the period of investigation with the exception of the month of June. Fifty to 86 animals per sampling were investigated. Highest prevalence and infection intensities were seen in September. A correlation between epitheliocystis infection and water temperatures was not evident. Interyear comparison revealed consistent levels of prevalence and infection intensities in late summer. The absence of infections in June, combined with the consistent interyear results, indicates seasonal fluctuation of epitheliocystis infections in brown trout with a reservoir persisting during winter months from which infections can re‐initiate each year. This could either be at levels below detection limits within the brown trout population itself or in an alternative host. 相似文献
103.
M. Akhlasur Rahman Isaac Kofi Bimpong J. B. Bizimana Evangeline D. Pascual Marydee Arceta B. P. Mallikarjuna Swamy Faty Diaw M. Sazzadur Rahman R. K. Singh 《Rice》2017,10(1):47
Background
Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.Results
The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.Conclusions
The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.104.
The efficiency of nitrogen in cattle manures when applied to a double‐annual forage cropping system 下载免费PDF全文
B. Perramon À. D. Bosch‐Serra F. Domingo‐Olivé J. Boixadera 《Grass and Forage Science》2017,72(4):676-690
The use of cattle manure (CM) for fertilization presents challenges for optimizing nitrogen (N) use. Our work aimed to assess N efficiencies, in a 6‐year experiment with three biennial rotations of four crops: oat–sorghum (first year) and ryegrass–maize (second year) in a rainfed humid Mediterranean area of Spain. Fertilization treatments included the following: control (no N), 250 kg mineral N ha?1 year?1 (250MN), three CM rates (supplying 170, 250 and 500 kg N ha?1 year?1) and four treatments where the two lowest CM rates were complemented with either 80 or 160 kg mineral N ha?1 year?1. Treatments were distributed randomly in each of three blocks. Maximum dry‐matter yield (~44–49 t ha?1 rotation?1) was achieved in the third rotation, and only the control and the 170CM yielded significantly less. Within the limitations of the EU Nitrate Directive, the N steady state supply of 170CM always requires a complement of mineral N (80 kg N ha?1) to maximize N agronomic efficiency. The maximum N‐fertilizer replacement value (250CM vs. 250MN) was 0·67, without significant differences between the two treatments in other N‐related efficiency indexes, which indicates that plants took advantage of residual‐N effects. Nitrogen losses by leaching in the 250CM treatment were around 5–7% of the N applied. This reinforces the sustainability of manure recycling in long cropping seasons. 相似文献
105.
Effect of l‐ascorbyl‐2‐polyphosphate supplementation on growth performance,body composition,antioxidative capacity and salinity stress tolerance of juvenile Pacific white shrimp,Litopenaeus vannamei 下载免费PDF全文
Shi‐Jun Chen Yu‐Jie Gao Shi‐Wei Xie Jin Niu Fan Yang Wei‐Ping Fang Li‐Xia Tian Yong‐Jian Liu 《Aquaculture Research》2017,48(9):4608-4622
An 8‐week feeding trial was conducted to evaluate the effects of ascorbic acid (AsA), in the form of l ‐ascorbyl‐2‐polyphosphate (LAPP) on growth performance, body composition, antioxidative capacity and salinity stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Five practical diets (46% crude protein and 7.6% lipid) supplemented with graded levels of AsA (14.64, 48.55, 84.98, 308.36 and 639.27 mg kg?1 diet) were fed to five replicate groups of L. vannamei (mean initial wet weight 0.57 g). No significant differences were found on growth performance among all treatments. However, whole body lipid content significantly decreased with dietary AsA levels increasing. Activities of total antioxidant capacity, glutathione reductase and glutathione peroxidase were significantly affected by dietary AsA levels. Shrimp fed LAPP‐free diet had higher malondialdehyde content than those fed the diets supplemented with LAPP. Dietary AsA levels higher than 308.36 mg kg?1 diet increased the survival of shrimps after 1, 2 and 3 h of acute salinity change. Broken‐line regression analysis on survival after 3 h of salinity stress and second‐degree polynomial regression analysis on glutathione reductase data indicated that the optimal dietary AsA requirement of L. vannamei was estimated to be 306.39, 319.75 mg kg?1 diet respectively. 相似文献
106.
Herbage production,nutritive value and animal productivity within hardwood silvopasture,open and mixed pasture systems in Appalachia,United States 下载免费PDF全文
Demand for livestock food products is projected to increase dramatically through to 2050. Increased livestock production capacity on marginal lands will be critical to meeting this demand. A 5‐year research effort was undertaken to evaluate lamb and sward productivity within open and hardwood silvopasture (SP) systems in Appalachia, USA. Grazing began in mid to late April each year, with the grazing season averaging 141 d. Grazing system treatments during 2002 and 2003 grazing seasons were as follows: 100% open pasture (OP), 67% OP and 33% SP, and 67% OP and 33% SP with delayed SP grazing initiation (OSD). In 2004, a 100% SP (SP) system was added. Animals were rotationally stocked through either 6 (2002–2004) or 7 (2005–2006) paddocks. Open pasture produced greater (P < 0·001) grazing season herbage yield, while all systems generated similar animal performance. Based on summer solstice, herbage production in spring was greater (P < 0·001) than summer, except in 2003. Total non‐structural carbohydrate (TNC) content was greater (P < 0·05) in spring than in summer, except in 2004. Animal performance was superior in spring versus summer (P < 0·001). Animal plasma urea nitrogen (PUN) was lower (P < 0·05) for OP in 2003. When PUN was correlated with nutritive value indicators, the ratio of TNC to crude protein (CP) had the strongest correlation. The strong correlation indicates the need for synchronized ruminal energy and CP availability. Development of silvopasture from existing woodlots has potential to improve whole farm productivity on marginal lands. 相似文献
107.
Aphanomyces astaci presence in Japan: a threat to the endemic and endangered crayfish species Cambaroides japonicus? 下载免费PDF全文
Agata Mrugała Tadashi Kawai Eva Kozubíková‐Balcarová Adam Petrusek 《水产资源保护:海洋与淡水生态系统》2017,27(1):103-114
- Spread of the crayfish plague pathogen, Aphanomyces astaci, by North American crayfish species is considered one of the main reasons for substantial declines and local extinctions of native European crayfish populations. Owing to human introductions, several American crayfish species have become established throughout the world, and thus pose a potential threat to indigenous crayfish populations susceptible to crayfish plague.
- In Japan, two such widespread alien species, Procambarus clarkii and Pacifastacus leniusculus, were introduced for aquaculture purposes in the late 1920s and since then successfully expanded their ranges.
- Aggressive interactions with alien crayfish along with habitat modifications have been considered primarily responsible for drastic declines in populations of the Japanese endemic crayfish, Cambaroides japonicus, observed in the last few decades. However, the presence of the crayfish plague pathogen, to which Japanese crayfish are susceptible, may be expected, and could contribute to these declines.
- Only recently, A. astaci has been reported from Taiwan, and to our knowledge no study focusing on its presence outside of the Western Palearctic has been conducted.
- To fill this gap, 54 P. clarkii and 47 P. leniusculus individuals from five different Japanese locations were screened using molecular methods recommended by the World Organization for Animal Health. Aphanomyces astaci DNA was detected in all studied populations, altogether in 61% and 21% of examined individuals of P. clarkii and P. leniusculus, respectively.
- The results provide the first evidence of A. astaci presence in Japan and highlight the threat of pathogen transmission to C. japonicus populations.
108.
A 7‐week growth trial was conducted to evaluate the effects of dietary soybean meal (SBM) on digestive enzyme activity of intestinal mucosa, mRNA levels of digestive enzymes in hepatopancreas, and the mid‐intestinal and hepatopancreas histology of gibel carp CAS III (Carassius auratus gibelio). Four different growth phases of gibel carp (initial body weight: fry, 0.8 g; juvenile, 5.0 g; 1‐year‐old, 62.7 g; and broodstock, 135.6 g) were tested. Seven isonitrogenous and iso‐energetic diets were formulated to contain different SBM replacement levels (0%, 20%, 40%, 60%, 80% and 100% of dietary fish meal protein), and another diet (SBMAA) contained all SBM protein and supplied crystalline amino acids. The results showed that the activities of mid‐intestine trypsin, α‐amylase and gamma glutamyl transpeptidase reduced with increased dietary SBM, while the chymotrypsin activity increased first and then decreased. The ultrastructures of intestinal epithelial cells and hepatopancreas cells in fry and broodstock fish were distinctly affected by 200 g kg‐1 dietary SBM. Supplementation of dietary amino acid to the highest replacement groups was not sufficient to improve digestive and absorptive capacities and growth performance. Gibel carp may be adapted to dietary SBM through increase in gene expression of hepatopancreas digestive enzymes and has potential to utilize proceeded SBM as feedstuffs. 相似文献
109.
S.H.S. Dananjaya D.M.S. Munasinghe H.B.S. Ariyaratne J. Lee M. De Zoysa 《Aquaculture Nutrition》2017,23(2):255-263
Discovering natural carotenoids for colour enhancement and health benefits of fish is important to develop new feed formulations. We have purified natural bixin from achiote seeds and evaluated the effect of colour enhancing and pigmentation in goldfish. Varying levels of bixin‐based diets were prepared with 420 g kg?1 of crude protein and 120 g kg?1 of lipid content. Our results clearly showed that bixin (0.05, 0.10, 0.20 and 0.60 g kg?1) based diets significantly (P < 0.05) enhanced the skin and fin colour at 30 and 60 days compared to diet without bixin. Interestingly, diet which contains 0.20 g kg?1 bixin and commercial feed (with astaxanthin) had similar effect on carotenoid deposition in skin. Moreover, total carotenoid deposition in fin was higher than in skin of all bixin‐containing diets. However, 0.60 g kg?1 bixin‐containing diet had lower specific growth rate (1.01 ± 0.01) and higher feed conversion ratio (2.05 ± 0.19) compared to the control group. The present results demonstrate that achiote bixin can be successfully used as an alternative natural carotenoid source against synthetic astaxanthin in fish feed. Our data indicate that 0.20 g kg?1 is a suitable dietary level of bixin to ensure strong pigmentation, acceptable growth and feed utilization in goldfish. 相似文献
110.
N.‐N. Zhang Q.‐Q. Ma W.‐J. Fan Q. Xing Y.‐L. Zhao L.‐Q. Chen J.‐Y. Ye M.‐L. Zhang Z.‐Y. Du 《Aquaculture Nutrition》2017,23(2):313-321
Oriental river prawn (Macrobrachium nipponense) has been widely cultured in Asian countries. However, its nutritional studies are very limited. In the present 8‐week study, we investigated the effects of dietary protein to energy ratio (P/E ratio) on the growth, feed utilization and body composition in juvenile M. nipponense (initial weight 0.302 ±0.03 g). Two‐factor experiment was designed and nine semi‐purified diets were formulated to contain three lipid levels (20, 80 and 140 g kg?1) and three protein levels (330, 380 and 430 g kg?1), producing P/E ratios from 16.5 to 23.4 mg KJ?1 protein. The results indicated that the growth, survival rate and protein efficiency were dose dependently improved by the increased dietary lipid, but not dietary protein content. Increased dietary lipid content and/or protein content increased lipid accumulation in whole body, hepatopancreas and muscle, but did not change the feed intake and hepatopancreas weight. In conclusion, our present study indicated that M. nipponense is a species with relatively high‐energy requirement. It could utilize dietary lipid content up to 140 g kg?1, while the dietary protein with more than 330 g kg?1 would not promote growth and protein efficiency. Taken together, 330 g kg?1 dietary protein and 140 g kg?1 dietary lipid level with P/E ratio 16.49 could be optimum for M. nipponense. 相似文献